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For GSIS experiments, approximately 30 islets were exposed to 2.8 mM glucose, 20 mM glucose or 20 mM glucose and 50 nM exendin-4 (Sigma, E7144) in Krebs-Ringer buffer supplemented with HEPES (Sigma, 83264) and 0.5% fatty-acid free BSA (Sigma, A7030)

Moreover, it is characterized by a high prevalence of coexistence with many other diseases including metabolic disorders such as type 2 diabetes mellitus (T2DM) and obesity

Exp Toxicologic Pathol (2012) 64:5038

Heavy meals close to bedtime: Conversely, if you havent managed your calorie intake well during the day and find yourself eating a large, heavy meal just before bed, your body will be working hard to digest it